Investigating the changes in streamflow regimes is useful for understanding the mechanisms associated with hydrological processes in different watersheds and for providing information to facilitate water resources management. In this study, we selected three watersheds, i.e., Sandu River, Hulu River, and Dali River on the Loess Plateau, to examine the changes in the streamflow regimes and to determine their responses to different soil and water conservation measures (terracing, afforestation, and damming). The daily runoff was collected continuously by three hydrological gauges close to the outlets of the three watersheds from 1965 to 2016. The eco-surplus, eco-deficit, and degree of hydrological change were assessed to detect hydrological alterations. The Budyko water balance equation was applied to estimate the potential impacts of climate change and human activities on the hydrological regime changes. Significant decreasing trends (P < 0.05) were detected in the annual streamflow in the Sandu and Dali River watersheds, but not in the Hulu River watershed where afforestation dominated. The annual eco-surplus levels were low and they decreased slightly at three stations, whereas the eco-deficit exhibited dramatic increasing trends in the Sandu and Dali River watersheds. In the Sandu River watershed (dominated by terraces), the runoff exhibited the most significant reduction and the eco-deficit was the highest among the three watersheds. The integral degrees of hydrological change were higher in the Sandu River watershed than the other two watersheds, thereby suggesting substantial variations in the magnitude, duration, frequency, timing, and rate of change in the daily streamflow. In the Dali River watershed (dominated by damming), the changes in the extreme flow were characterized by a decreasing number appearing in high flow. In these watersheds, human activities accounted for 74.1% and 91.78% of the runoff reductions, respectively. In the Hulu River watershed (dominated by afforestation), the annual runoff exhibited an insignificant decreasing trend but with a significant increase in the low flow duration. Rainfall changes accounted for 64.30% of the runoff reduction.