not-yet-known not-yet-known not-yet-known unknown Drought is a critical risk factor that impacts rice growth and yields. Previous studies have focused on the regulatory roles of individual transcription factors in response to drought stress. However, there is limited understanding of multi-factor stresses gene regulatory networks and their mechanisms of action. In this study, we utilized data from the jaspar database to compile a comprehensive dataset of transcription factors and their binding sites in rice, Arabidopsis, and barley genomes. We employed the pytorch framework for machine learning to develop a 9-layer convolutional deep neural network TFBind; its accuracy was 90% through literature review and dual-luciferase assay validation. Subsequently, we obtained rice RNA-seq and ATAC-seq data related to abiotic stress from the public database. Utilizing integrative analysis of WGCNA and ATAC-seq, we effectively identified transcription factors associated with open chromatin regions in response to drought. Interestingly, only 81% of the transcription factors directly bound to the opened genes by testing with TFBind model. By this approach we identified 15 drought-responsive transcription factors corresponding to open chromatin regions of targets, which enriched in the terms related to protein transport, protein allocation, nitrogen compound transport. This approach provides a valuable tool for predicting TF-TAG modules during biological processes.