Chronic spontaneous urticaria (CSU) is defined as the recurrent episodes of spontaneous wheals and/or angioedema for more than 6 weeks, and at least twice a week. The core link in the pathogenesis of CSU is the activation of mast cells and other immune cells caused by various reasons. These activated immune cells release a series of inflammatory active mediators such as histamine, arachidonic acid metabolites, chemokines, etc., resulting in clinical features such as wheals and/or edema. However, the specific mechanisms leading to the activation of various immune cells have not been fully elucidated. Previous evidence has shown that about 50% of CSU patients have potential autoimmune reactions. Here we review the functional implication of immune cells in CSU focusing on the crosstalk between them and discuss whether their crosstalk promotes the occurrence and development of the disease.