­­­­­­­­­­­­ Herbal medicines are non-toxic, low-cost and have no side effects. They are successful in treating a variety of ailments, but due to a lack of documentation and strict quality control, they remain unsatisfactorily. Daphne mucronata Royle belongs to Thymelaeaceae, proved to be a vital anticancer plant, yet there is no report on its standardization. Therefore, the study was made in order to fix the micromorphological and pharmacognostic standards for the leaf. Morpho-anatomical examination revealed useful information for the identification. The results of macromorphological gave a base for the use of leaf of D. mucronata which having gray green color, characteristic unlikable odor and unpleasant taste. The Scanning Electron Microscopy (SEM) and Light microscopy (LM), showed characteristics features of the leaf resembled with other family members. The SEM study revealed a unique crystal, which has never been seen before in the genus. The leaves surface features depicted ranunculacious, paracytic, and diacytic stomata. Extractive values determination, fluorescence, and UV analysis were carried out. The phytochemicals tests showed the presence of fats, terpenoids, cardiac glycosides, amino acids, saponins, tannins (70 %), reducing sugars, mucilages, phenolics (55 %), proteins, flavonoids (52.5 %), volatile oils, and starch. These phytochemicals are accountable for the pharmacological potential of this plant. Elemental analysis, nutritional analysis, and oil extraction was done following standards methods. These provide the standard parameters for the genuineness of the leaves. These informations will assist in the authentication and consistent quality, resulting in the safe use and preservation of this plants’ efficacy.