Grain size affects the rates of aeolian sediment transport on beaches. Sediment in coastal environments typically consists of multiple grain size fractions and exhibits spatiotemporal variations. Still, conceptual and numerical aeolian transport models are simplified and often only include a single fraction that is constant over the model domain. It is unclear to what extent this simplification is valid and if the inclusion of multi-fraction transport and spatial grain size variations affects aeolian sediment transport simulations and predictions of coastal dune development. This study applies the numerical aeolian sediment transport model AeoLiS to compare single-fraction to multi-fraction approaches for a range of grain size distributions and spatial grain size scenarios. The results show that on timescales of days to years, single-fraction simulations with the median grain size, D50, often give similar results to multi-fraction simulations provided the wind is able to mobilize all fractions within that time frame. On these timescales, vertical variability in grain size has a limited effect on total transport rates, but it does influence the simulation results on minute timescales. Horizontal grain size variability influences both the total transport rates and the downwind bed grain size composition. The results provide new insights into the influence of beach sediment composition and spatial variability on total transport rates towards the dunes. The findings of this study can guide the implementation of grain size variability in numerical aeolian sediment transport models.