Reconstructing atmospheric CO2 concentration in the Late Miocene is crucial for understanding the relationship between greenhouse gas concentrations and climate change in a warmer-than-modern world. Both δ11B-based and alkenone-ep-based CO2 estimates feature uncertainties due to poorly constrained past seawater chemistry, and algal physiological processes, respectively. Additionally, both proxies estimate CO2[aq], so they require reliable surface ocean temperatures to calculate solubility and atmospheric CO2. To evaluate proxy coherence, in this study we generate new records of alkenone ep and δ11B, from the western Tropical Atlantic ODP Site 926 during the Late Miocene. We provide surface ocean temperature estimates from coccolith clumped isotope thermometry, alkenone undersaturation ratios, and planktonic foraminiferal Mg/Ca ratios. The warm temperatures estimated from our new clumped isotope records, together with alkenone temperatures >29°C, confirm warm tropics, and provide constraints on the assumptions of seawater Mg/Ca and dissolution corrections for foraminiferal Mg/Ca SST estimates. The new alkenone ep CO2 estimates at 926 yield generally similar CO2 as the new and published δ11B-based CO2 records for the site, and are similar to published alkenone ep CO2 records from the South Atlantic ODP Site 1088. However, over the 7.3 to 7.8 Ma interval, the CO2 values from ep are lower than other records. We evaluate which proxy indicators can best predict variations in algal physiology which may bias the ep-based CO2 reconstructions in this interval at Site 926.

Large Antarctic ice volume changes characterized the middle to Late Oligocene and the first million years of climate evolution during the Miocene. However, the sea surface temperature (SST) evolution over this period remains poorly constrained, as only a few records from contrasting proxies are available. In this study, we present a long-term alkenone-derived SST record from sediments drilled by the Ocean Drilling Program (ODP) at Site 1168 in the west Tasmanian Sea spanning 29.8 Ma to 16.7 Ma. The SST record highlight that the long-term warming in the Late Oligocene linked to the end of the Middle Oligocene Glacial Interval can be recognized also at mid-to-high latitudes of the Southern Hemisphere. Warmer average temperatures (25.5°C) characterize the period from 24.6 to 22 Ma; average temperatures then decrease by 1 to 2°C into the Miocene and stabilize by 20.1 Ma. The reconstructed temperatures are highly variable in the warm Late Oligocene waters, and more stable and slightly colder in the Early to Middle Miocene. We confirm that this temperature trend is not an artefact of the latitudinal drift of the site, as the temperature anomaly relative to the modern water temperature at the paleolocation confirms the SST trends of the Oligocene. This is the first alkenone-derived record to reproduce the long-term Oligocene climate trend previously interpreted from the benthic δ18O, which recorded a warming and/or reduction in ice volume from the Middle Oligocene Glacial Interval through the latest Oligocene.