The stable carbon isotope composition (δ13C) of plant components such as plant wax biomarkers is an important tool for reconstructing past vegetation. Plant wax δ13C is mainly controlled by photosynthetic pathways, allowing for the differentiation of C4 tropical grasses and C3 forests. Proxy interpretations are however complicated by additional factors such as aridity, vegetation density, elevation, and the considerable δ13C variability found among C3 plant species. Moreover, studies on plant wax δ13C in tropical soils and plants have focused on Africa, while structurally different South American savannas, shrublands and forests remain understudied. Here, we analyze the δ13C composition of long-chain n-alkanes and fatty acids from tropical South American soils and leaf litter to assess the isotopic variability in each vegetation type and to investigate the influence of climatic features on δ13C. Rainforests and open vegetation types show distinct values, with rainforests having a narrow range of low δ13C values (n-C29 n-alkane: -34.5 +0.9/-0.6 ‰ ; Suess-effect corrected) allowing for the detection of even minor incursions of savanna into rainforests (13C-enriched). While Cerrado savannas and semi-arid Caatinga shrublands grow under distinctly different climates, they can yield indistinct δ13C values for most compounds. Cerrado soils and litter show pronounced isotopic spreads between the n-C33 and n-C29 alkanes, while Caatinga shrublands show consistent values across the two homologues, thereby enabling the differentiation of these vegetation types. The same multi-homologue isotope analysis can be extended to differentiate African shrublands from savannas.

Sedimentary specimens of the planktonic foraminifera Globorotalia inflata can provide much needed information on subsurface conditions of past oceans. However, interpretation of its geochemical signal is complicated by possible effects of cryptic diversity and encrustation. Here we address these issues using plankton tow and sediment samples from the western South Atlantic, where the two genotypes of G. inflata meet at the Brazil-Malvinas Confluence Zone. The 18O and δ13C of encrusted specimens from both genotypes from a core within the confluence zone are indistinguishable. However, we do find a large influence of encrustation on δ18O and Mg/Ca. Whereas crust Mg/Ca ratios are at all locations lower than lamellar calcite, the crust effect on δ18O is less consistent in space. Plankton tows show that encrusted specimens occur at any depth and that even close to the surface crust Mg/Ca ratios are lower than in lamellar calcite. This is inconsistent with formation of the crust at lower temperature at greater depth. Instead we suggest that the difference between the crust and lamellar calcite Mg/Ca ratio is temperature-independent and due to the presence of high Mg/Ca bands only in the lamellar calcite. The variable crust effect on δ18O is more difficult to explain, but the higher incidence of crust free specimens in warmer waters and the observation that a crust effect is clearest in the confluence zone, hint at the possibility that the difference reflects advective mixing of specimens from warmer and colder areas, rather than vertical migration.

Intensification of the Agulhas Leakage (AL) during glacial terminations has long been proposed as a necessary mechanism for reverting the Atlantic Meridional Overturning Circulation (AMOC) to its interglacial mode. However, lack of records showing the downstream evolution of AL signal and substantial temporal differences between AL intensification and resumption of deep‐water convection have cast doubt on the importance of this mechanism to the AMOC. Here, we analyze a combination of new and previously published data relating to Mg/Ca‐derived temperatures and ice volume‐corrected seawater δ18O records (δ18OIVC‐SW, as a proxy for relative changes in ocean salinity), which demonstrate propagation of AL signal via surface and thermocline waters to the western South Atlantic (Santos Basin) during Termination II and the early Last Interglacial. The saline AL waters were temporally stored in the upper subtropical South Atlantic until they were abruptly released in two stages into the North Atlantic via surface and thermocline waters at ca. 129 and 123 ka BP, respectively. Accounting for age model uncertainties, these two stages are coeval with the resumption of convection in the Labrador and Nordic seas during the Last Interglacial. We propose a mechanism whereby both active AL and a favorable ocean‐atmosphere configuration in the tropical Atlantic were required to allow flux of AL waters into the North Atlantic, where they then contributed to enhancing the AMOC during the Last Interglacial period. Our results provide a framework that connects AL strengthening to the AMOC intensifications that followed glaciations.