Huiyong Hu

and 4 more

Huiyong Hu1#, Xiaoping Jing2#, Xiuhua Duan3, Leiping Zhou4, Yunfeng Xu1*1 Department of the Ultrasonography, Shanghai Children’s Hospital, Shanghai Jiao Tong University, school of medicine, Shanghai 200040, China;2 Department of Traditional Chinese Medicine, Shanghai Children’s Hospital, Shanghai Jiao Tong University, school of medicine, Shanghai 200040, China;3 Department of Radiology, Shanghai Children’s Hospital, Shanghai Jiao Tong University, school of medicine, Shanghai 200040, China;4 Department of Radiology, International Peace Maternity & Child Health Hospital of China welfare institute, Shanghai Jiao Tong University, school of medicine, Shanghai 200030, China;# These authors contributed equally to this work.* Corresponding author: Yunfeng Xu, Department of the Ultrasonography, Shanghai Children’s Hospital, Shanghai Jiao Tong University, school of medicine;Address: 1400 West Beijing Road, Shanghai, China Lane 24 Zip Code 200040;Phone: 18917128478E-mail: xuyunfeng65@163. com (F X).During a prenatal ultrasonography examination late in the second trimester, a fetus was found to have a right diaphragmatic hernia (Figure S1). Multidepartment dynamic monitoring was instituted, and the fetus was later successfully delivered by cesarean section after fetal distress became evident. After intubation, the infant was stabilized and transferred to the Department of Neonatology at our hospital.The enhanced computed tomography of the chest and stomach displayed multiple air-filled intestinal shadows in the right chest cavity, the widest being about 20.0 mm. The right lung, mediastinum, and heart were compressed and displaced, and most of the lung tissue in the right lung was consolidated. Atelectasis is evident in the irregular enhancement shadow at the right upper abdomen, about 43.5 × 32.0 mm in size. The boundary between some sections and the posterior margin of the right lobe of the liver was unclear, but the blood supply (hepatic artery and portal vein branches) was visible (Figure). Blood gases, routine bloodwork, liver and kidney function, and myocardial enzymes were essentially normal.At 40 + 4 weeks, with the infant under total anesthesia, hernia repair was performed. The liver and intestines in the thoracic cavity were brought back into the abdominal cavity; the tissues around the hernia ring in the diaphragm were carefully dissociated; and patch repair and suturing were performed (Figures S2–S4). After the operation, the infant’s vital signs were stable and their condition remained good during follow-up.Congenital diaphragmatic hernia (CDH) is a potentially fatal birth defect[1-3]. In China today, all pregnant women undergo ultrasonography to uncover pregnancy- related conditions[4]. A “green channel” – that is, a multidepartment collaborative for the emergency treatment of perioperative pulmonary hypertension, pulmonary dysplasia, and other complications in newborns with CDH – has been established, helping to assure the best prognosis for those infants.

Jing wang

and 5 more

This investigation was to confirm the relationship between asthma, respiratory syncytial virus (RSV) infection, and the gut environment by analyzing the alterations in the gut microbiota of RSV-infected asthmatic mice. All BALB/c male mice were randomly separated into the control group (CON), ovalbumin (OVA) group, and OVA + RSV group (n = 8). At the end of experiment, we evaluated the RSV-infected asthma model using Wright-Giemsa staining, immunoglobulin E (IgE) levels using ELISA, and inflammatory cells using Hematoxylin and eosin (HE). Furthermore, airway hyperresponsiveness (AHR) was measured by a Buxco’s modular and invasive system. Histopathological analysis of the murine lung tissue revealed that the inflammatory infiltration, edema, and collagen hyperplasia were more severe in the OVA + RSV group than in the other groups. Higher expression of interleukin-5 (IL-5), IL-13, IL-25, and IL-33 in mice from the OVA and OVA + RSV groups (*P < 0.05 and **P < 0.01). Moreover, feces were collected for 16S rRNA sequencing and data analysis. The associations between Prevotellaceae_UCG_001, which is positive, and immunoglobulin E (IgE), IL-13, and IL-33 were highly significant (***P < 0.001), IL-5 (**P < 0.01) and IL-25 (*P < 0.05). Lachnospiraceae_NK4A136_group was also positive, and significantly associated with IgE and IL-33. Helicobacter and Uncultured_Bacteroidales_bacterium are negative, had associated with IL-25 (*P < 0.05). Thus, we conclude that asthma with RSV infection can alter the major components of gut microbiota and influence the mutual relationship between the core operational taxonomic units (OTUs) and IgE as well as inflammatory cytokines.