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Oxygen uptake rate (OUR) guided nitrogen control strategy for improving nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus
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  • Xiaoqing Song,
  • Zishu Zhang,
  • Junxiong Yu,
  • Yun Zhang,
  • Jiayun Xue,
  • Yuanxin Guo,
  • Jianguang Liang,
  • Ming Ren,
  • Qingyun Ling,
  • Ali Mohsin,
  • Jiangchao Qian ,
  • Zejian Wang,
  • yonghong wang
Xiaoqing Song
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Zishu Zhang
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Junxiong Yu
East China University of Science and Technology School of Biotechnology
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Yun Zhang
East China University of Science and Technology School of Biotechnology
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Jiayun Xue
East China University of Science and Technology School of Biotechnology
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Yuanxin Guo
East China University of Science and Technology School of Biotechnology
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Jianguang Liang
Changshu Institute of Technology
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Qingyun Ling
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Ali Mohsin
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
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Jiangchao Qian
East China University of Science and Technology School of Biotechnology
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Zejian Wang
East China University of Science and Technology

Corresponding Author:[email protected]

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yonghong wang
East China University of Science and Technology
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Abstract

Oxygen supply and oxygen uptate rate (OUR) significantly influences both the physiological state of cells and nemadectin biosynthesis by Streptomyces cyaneogriseus ssp. noncyanogenus. In this study, a real-time monitoring approach for nemadectin fermentation was developed for the first time by an on-line OUR control strategy. The effect of OUR levels during the mycelium differentiation phase on nemadectin biosynthesis was studied and optimized by adjusting feeding rates of (NH4)2SO4. Results showed that controlling the OUR at approximately 17.5 mmol·L-1·h-1 during the mycelium differentiation phase effectively enhanced nemadectin production, reaching 2805.1 μg/mL, making 129.5% increase compared to the control. This OUR control strategy was successfully scaled-up to a 500 L pilot scale, achieving the highest ever nemadectin production of 2867.3 μg/mL. These findings demonstrate that this OUR control strategy provides an effective and scalable approach for industrial nemadectin production.