loading page

Targeted proteomic analysis of sickle cell disease patients with elevated tricuspid regurgitation velocity
  • +7
  • Varshini Babu,
  • Jane Little,
  • Claudia Morris,
  • Robert Machado,
  • Simon Gibbs,
  • Gregory Kato,
  • Victor Gordeuk,
  • Mark Gladwin,
  • Seyed Nouraie,
  • Yingze Zhang
Varshini Babu
University of Pittsburgh

Corresponding Author:[email protected]

Author Profile
Jane Little
The University of North Carolina at Chapel Hill
Author Profile
Claudia Morris
Emory University School of Medicine
Author Profile
Robert Machado
Indiana University
Author Profile
Simon Gibbs
Imperial College London
Author Profile
Gregory Kato
CSL Behring LLC
Author Profile
Victor Gordeuk
University of Illinois Chicago
Author Profile
Mark Gladwin
University of Pittsburgh
Author Profile
Seyed Nouraie
University of Pittsburgh
Author Profile
Yingze Zhang
University of Pittsburgh
Author Profile

Abstract

Purpose: Pulmonary hypertension (PH) is a chronic complications of sickle cell disease (SCD) with limited known biomarkers, beyond increases in plasma brain natriuretic peptide levels. Experimental Design: We conducted a proof-of-concept study to identify serum protein biomarkers that were differentially expressed in SCD patients with elevated tricuspid regurgitation velocity (TRV – a noninvasive marker of PH). Results: We found 41 out of 92 target proteins that were significantly different between the non-elevated (TRV≤2.6 m/sec; N = 35) and highly elevated TRV group (TRV≥2.9 m/sec; N = 35, p<0.05). Six of them passed a Bonferroni correction (p value < 0.0005) including T-cell surface glycoprotein, lymphotactin, SLAM family member 7, galectin-9, TNF-related apoptosis-inducing ligand receptor 2, and tumor necrosis factor receptor superfamily member 11A . We observed up to a 1.2-fold increase in the high TRV group for these six proteins. These six proteins had strong positive correlation with serum NT-proBNP levels (a positive control marker elevated in PH (r >= 0.44). Additionally, these markers correlated with other clinical parameters of PH in SCD. Conclusion: Circulatory protein markers of the immune response are increased in SCD patients with elevated TRV as compared to those without elevated TRV.
19 Jul 2024Submitted to Clinical Applications
23 Jul 2024Submission Checks Completed
23 Jul 2024Assigned to Editor
23 Jul 2024Review(s) Completed, Editorial Evaluation Pending
25 Jul 2024Reviewer(s) Assigned