Effective management of water resources and mitigation of water-related disasters are essential for human well-being, ecosystem health, and socio-economic development. However, the challenge lies in translating scientific insights into actions and decisions, resulting in a disconnect between knowledge production and practical decision-making. SERVIR, a collaborative initiative by the U.S. Agency for International Development (USAID) and the U.S. National Aeronautics & Space Administration (NASA), addresses this gap by partnering with regional organizations worldwide to integrate Earth Observation (EO) data into practical applications. This paper evaluates SERVIR’s experience translating cutting-edge science into actionable information for water security/water resources management through a collaborative approach, co-developing tools within diverse cultural contexts, and emphasizing capacity building. By discussing case studies and engagement strategies from SERVIR’s extensive experience, we highlight its collaborative efforts with regional bodies, governmental agencies, and other partners to transform water resources research into practical insights, supporting decision-making at various levels. This paper underscores the importance of continuous capacity-building workshops, stakeholder engagement, and adapting to technological advancements, such as cloud computing, for sustained impact. It also addresses the need for effective translators to navigate the complex EO toolkit, ensuring the appropriate application of tools for specific water management decisions. By reflecting on SERVIR’s journey, this paper offers guidance for decision-makers, practitioners, and researchers, encouraging dialogue, innovation, and collective action to support reliable access to water for all and sustainable water management. The insights and opportunities derived from SERVIR’s experience provide a framework for future initiatives, advancing water security and water resource management strategies.