1. Invasive alien species constitute a major threat for aquatic systems due to their potential impact on endemic biodiversity, ecosystem functioning, infrastructure, and possible sanitary issues. It is therefore crucial to obtain information on their presence, abundance and distribution. 2. The red blood mysid Hemimysis anomala, which originated from the Ponto Caspian area, has recently settled in western European lakes, including Lake Geneva. 3. Although divers have frequently reported the presence and development of this small crustacean over the last decade, no monitoring has yet been proposed. 4. During a period of 2.5 years, we tested and optimized an eDNA approach by comparing two PCR techniques, quantitative and digital droplet PCR, to assess the presence, abundance and dynamics of the animal as well as that of a potential predator, the perch (Perca fluviatilis). 5. We show and discuss the efficiency of the methods and reveal for the first time the seasonal dynamics of Hemimysis anomala at a selected site in Lake Geneva. We highlight, in agreement with diving observations that the animal’s abundance is high in winter and declines rapidly in early spring, concomitantly with temperature increase and perch presence.