Abstract
Immunization has been considered as a successful global health program
that saves life of many persons each year. The vaccines reduce the risk
of getting the disease by providing immunity in the body. Therefore, the
constant availability of essential vaccines is an important factor in
the health of the community. One of the most important vaccines
worldwide is the diphtheria vaccine, which is usually given multivalent
with tetanus and pertussis. It takes about 45 days to produce this
vaccine from the time of initial culture to the production of the
toxoid. If it is possible to carry out many stages of production under
normal conditions and maintain the chemical intermediate, it will be a
great help to produce this vaccine. In this study, the method of
purification (by washing and chromatography) and storage of toxins for
several months is presented. In this method, by concentrating the
sample, it can be stored in a smaller space and there will be no problem
in providing a suitable space. Another problem with the diphtheria
vaccine is that it is reversible after detoxification of the toxin using
formaldehyde. In order to solve this problem, it is suggested to use
mPEG for detoxification, which will produce more stable covalent bonds
between PEG and the first type of amine groups (-NH2) in the toxin
chain. Therefore, we will have a stable product that the results of
animal tests show that this product caused no wound or necrosis in the
tested animals.