2.4 Detection of HPMEC ultrastructure, function and cell activity
Transmission electron microscopy (TEM) Cells were fixed in 1% osmium tetroxide (Sinopharm Chemical Reagent Co., Ltd. China) for 2 h, evaporated with a scalar series of acetone washes, infiltrated with propylene epoxied, and embedded in Epon 812. Further, 60–80-nm-thick sections were performed, and uranium acetate saturated with alcohol solution and lead citrates were dying respectively for 15 minutes. TEM (HT7700, HITACHI) was used to observe and image ultrastructures of HPMEC.