3.2 Widespread m6A methylation of rice genomic
mRNA with BPH infestation
The genomic mRNA m6A methylation modifications and
genes expression of un-infested (Nip) and BPH-infested Nip rice (Nl-Nip)
samples were compared using Nanopore Direct RNA Sequencing (DRS) and
Next Generation RNA Sequencing (NGS) respectively (Supporting
Information: Figure S1). Reads distribution analysis showed that all
samples exhibited considerable m6A methylation
enrichment within CDS and 3′-UTR regions (Supporting Information: Figure
S3). At the genomic level, these unique m6A-methylated
positions for each treatment were unevenly distributed across each rice
chromosome (Figure 1e, Supporting
Information: Table S1). Circo plots of the m6A
methylome in rice genomic RNAs showed that m6A
distribution density was highly consistent with the corresponding gene
density on the same chromosome position in the un-infested sample
(Figure 1e).