Fig. 3: MUC2 protein glycosylation in homeostatic (normal) gut environment versus disease/dysbiotic gut environment. A. MUC2 protein domain organization. The high density of O-glycosylation takes place in PTS domains. B. AlphaFold depiction of MUC2 region from residues 1260 to 1460 (UniProt Primary Accession: Q02817). Protein structure is colored according to model confidence. Visualization of the predicted aligned error plot demonstrates high model confidence between residues 1300-1395, associated with the ordered domain highlighted in dark and light blue. Model confidence falters in regions associated with PTS-repeats, indicative of the inherent disordered state of the protein backbone and displayed in orange and yellow. MUC2 glycans observed in a homeostatic (normal) gut environment are found to be densely packed. Glycan structures are elongated, sulfated, and fucosylated. MUC2 glycans observed in disease/dysbiotic gut environment are found to be sparser. Glycan structures are shorter, altered, and aberrant with increased expression of T antigen, Tn antigen, and STn antigen134,156,176,177,288,289.