Figure 4. Chemogenetic stimulation of the RCA and SCN promote arousal
Selective cases that outline AAV-M3-mCherry injection in the RCA (A). EEG/EMG and sleep-wake stage change after saline or CNO injection at 8:00 (B). Effects of RCA activation on NREM sleep latency, sleep-wake episodes transition, total number and mean duration of waking, NREM, and REM bouts during 8:00-11:00 (C and D). Selective cases that outline AAV-M3-mCherry injection in the SCN (E). EEG/EMG and stage changes saline or CNO injection at 8:00 (F). Effects of SCN activation on NREM sleep latency (G).