Figure 1. Chemogenetic stimulation of the PVH and PVH-PB pathway
promotes arousal.
Outlines of AAV-M3-mCherry transfection in the PVH in 8 cases (A),
EEG/EMG recording and corresponding hypnograms (8:00-11:00) in a rat
with saline and CNO injection (B) and Fos induced by CNO and mCherry
labeled neurons in the PVH (C), and effect of CNO on NREM sleep latency,
sleep-wake episodes transition, total number and mean duration of
waking, NREM, and REM bouts in a 6 h period (D-E). AAV6-cre injected in
the PB was labeled by cre (F1) while CNO induced Fos in mCherry neurons
were seen in the PVH where cre dependent AAV-M3-mCherry was injected
(F2-F3). EEG/EMG recordings and corresponding hypnograms in a rat with
activation of PVH-PB circuit by CNO (G). Effect of CNO on NREM sleep
latency, sleep-wake episodes transition, total number and mean duration
of waking, NREM, and REM bouts in a 2 h period (H).