Figure 2. Characterizations of sleep-wake effects by PVH chemo-activation
CNO injection at 8:00 and 20:00 increased wake amounts and reduces sleep in hourly time-course (A and C), and averaged amounts of 6,12, 12 hours (B and D). Each circle represents the hourly mean ± SEM. *P<0.05, **P<0.01. No sleep rebound is seen after CNO injection of 8:00 and 20:00.