Image Acquisition and Viral Expression Analysis:
For each animal, cannula placement and viral expression were analysed
using a bright field and fluorescent microscope Leica DM5000B, equipped
with a DFC310 FX camera. The viral expression was assessed at the
injection site, as well as at dorsal subiculum efferent targets. These
targets included layers 2 and upper 3 of the retrosplenial cortex, along
with the anteroventral and anteromedial thalamic nuclei (Figures 4, 5).