Aim Research Area Mitochondrial Genome Region Primer Name Forward Primer(5’-3’)
Reverse Primer(5’-3’) Length Reference
Threatened Species (Latonia nigriventer)
Northern Israel
12S
/
(5’- GAACTACGAGCCTCAGCTTAAA-3’) (5’- GGCAAGAAGTGGTGAGGTTA-3’)
110 bp
(Renan et al., 2017)
Threatened Species (Ambystoma cingulatum, Ambystoma bishopi)
Southeastern United States
D-loop
Amcin F Amcin R Amcin P (5’-GGCCCGTCAACTTTCCTCTAA-3’) (5’-TGGTCCAGGTAAATCAATTGCA-3’) (6FAM-TACGGTAATATGTCTGGTACTAC-MGBNFQ)
132 bp
(Anna M McKee et al., 2015)
Threatened Species (Notophthalmus perstriatus)
NADH Dehydrogenase Subunit 1 (ND1)
Noper F Noper R Noper P (5’-CCGAGCCCCCTTCGAT-3’) (5’-CTTCCTGCATATTCTACGTTAAATCCT-3’) (6FAM-TAACAGAAGGTGAATCTG-MGBNFQ)
71 bp
Threatened Species (Lithobates capito)
NADH Dehydrogenase Subunit 2 (ND2)
Licap F Licap R Licap P (5’-CGGCACTACAGTCACCCTATC-3’) (5’-TCGGGATAATGGCGAGGGTAT-3’) (CAL Fluor Red610-TYCATTGACTCTTAGCCTGAGTAGGCYTA-BHQ-2)
82 bp
Threatened Species (Phytotriades auratus) Trinidad, America
Cytb
GTF-F GTF-R (5’-CCCCTT ACATCGGCACTGAC-3’) (5’-CTCCAAGGATGTTTGGGGTGA‐3’)
390bp
(Brozio et al., 2017)
Threatened Species (Babina subaspera)
Amami-Oshima Island,Japan
ND5
BsubND5-F BsubND5-R (5’-TCACTCAATGCCCGCTTATATA-3’) (5’-GTACTCTAAAACCAATTTAGTA‐3’)
90 bp
(Takahara et al., 2020)
Threatened Species (Odorrana splendida)
ND5
OsplND5-F OsplND5-R (5’-GCTGAGAAGGCGTAGGATTT-3’) (5’-ACATCTCATGTCGAACATTGC‐3’)
96 bp
Threatened Species (Odorrana amamiensis)
ND5
OamaND5-F OamaND5-R (5’-TCGGCCCAGGCCCTTTTGAGCA-3’) (5’-TGATTGTGTTCCCACTA‐3’)
116 bp
Rare Species (Proteus anguinus)
Dinaric Karst, Croatia
D-loop
Paf8 Par8 (5’-GTGGCATATAAATCTATGTC-3’) (5’-TRTTATTCGTTTTCTAGG-3’)
64 bp
(Vörös et al., 2017)
Rare Species (Phytotriades auratus)
West Indies
Cytb
/
(5’- GCGGATTCTCTGTCGACAATG -3’) (5’- TGCTCCTGCAATAAGAAATGGA -3’)
80bp
(Torresdal et al., 2017)
Secretive Species (Ascaphus montanus)
Northwest United States
Cytb
Asmo F Asmo R (5’-CGTCAACTATGGCTGGCTAA-3’) (5’-TCGGCCAATGTGAAGATAAA-3’)
78 bp
(C. S. Goldberg et al., 2011)
Secretive Species (Dicamptodon aterrimus) Dicamp F Dicamp R (5’-TCTGCATCTTYCTACATATYGGAC-3’) (5’-ATCACYCCGACKTTTCAGGT-3’)
85 bp
Alien Invasive Species Quantitative Detection (Lithobates catesbeianus)
Western European
Cytb
BullfrogF BullfrogR (5’-TTTTCACTTCATCCTCCCGTTT-3’) (5’-GGGTTGGATGAGCCAGTTTG-3’)
84 bp
(T. Everts et al., 2021)
Alien Invasive Species Quantitative Detection (Lithobates catesbeianus) Western European Beijing, China
16S
qLC16S Forward qLC16S Reverse (5’-GCAGAGATAACCTCTCGT-3’) (5’-GTCCCATAGGACTGTTCT-3’)
120 bp
(T. Everts et al., 2021) (Lin et al., 2019)
Alien Invasive Species (Lithobates catesbeianus)
Southwest of France
Cytb
/
(5’-TGCCAACGGAGCATCATTC-3’) (5’-ATAAAGGTAGGAGCCGTAGT-3’)
79 bp
(Dejean et al., 2012)
Quantitative Detection Linkebeek, Belgium 12S 12S_F1 12S_R1 (5’-ACTGGGATTAGATACCCC-3’) (5’-TAGAACAGGCTCCTCTAG-3’) 142 bp (Brys et al., 2021)
Quantitative Detection (Ascaphus montanus) Idaho, United States
Cytb
qASMOF qASMOR (5’-ACGTCAACTATGGCTGGCTAATC-3’) (5’-GTCCTCGGCCAATGTGAAGA-3’)
within 200 bp
(Pilliod et al., 2013)
Quantitative Detection (Dicamptodon aterrimus) qDIATF qDIATR (5’-AAACTTTACACCAGCAAACCCATT-3’) (5’-CGGAGAATAGCGTATGCAAAAA-3’)
Species-specific detection (Hynobius kimurae)
Kyoto Prefecture, Japan
12S
Hynobius_12S_F1 Hynobius_12S_R1 Hida_12S_P (5’-TTAATAAAAACGGCCTAAAGCGTG-3’) (5’-TCAATTATAGAACAGGCTCCTCTAGGG-3’) ([FAM]-CCTTAAACTTTGGAGCCTACCCGCCTG-[TAMRA])
24~27 bp
(Jo et al., 2020)
Biodiversity survey Hainan Island,China 12S batra_F batra_R batra_blk (5’-ACACCGCCCGTCACCCT-3’) (5’-GTAYACTTATGTTACTT-3’) (5’-TCACCCTCCTCAAGTATACTTCAAAGGCA-SPC3I-3’) 150 bp (Li et al., 2021)
Biodiversity survey France 12S batra_F batra_R batra_blk (5’-ACACCGCCCGTCACCCT-3’) (5’-GTAYACTTATGTTACTT-3’) (5’-TCACCCTCCTCAAGTATACTTCAAAGGCA-SPC3I-3’) 20~60 bp (Valentini et al., 2016)
Biodiversity survey
California, United States
12S
12S-V5-F 12S-V5-R (5’-ACTGGGATTAGATACCCC-3’) (5’-TAGAACAGGCTCCTCTAG-3’)
106 bp
(W. E. Moss et al., 2022)
Biodiversity survey Brazilian Atlantic Forest 12S batra_F batra_R batra_blk (5’-ACACCGCCCGTCACCCT-3’) (5’-GTAYACTTATGTTACTT-3’) (5’-TCACCCTCCTCAAGTATACTTCAAAGGCA-SPC3I-3’) 52 bp (Sasso et al., 2017)