4. 9 Immunization and challenge experiment
Female C57BL/6 mice (6-8 weeks old) were intravenously injected with
three doses of salivary gland sporozoites at an interval of two weeks.
Control groups were injected with uninfected mosquito salivary gland
debris. Both groups were challenged with 5,000 WT sporozoites 10 post
last immunization. The appearance of parasites in blood was monitored by
making Giemsa-stained blood smears.