DNA logic programming, based on the principles of base complementarity and Boolean operations, exhibits organizational structures and computational rules similar to machine language. Consequently, DNA logic programming enables programming, decision-making, and output capabilities consistent with machine language in real-world applications. In recent years, the thinking behind DNA logic programming has synergized with life sciences, medicine, and various interdisciplinary fields, establishing an intelligent and digitized research framework. It has paved the way for cutting-edge interdisciplinary research and facilitated the transition of DNA logic programming from concept to construction. This article provides an overview of recent research achievements in the field of DNA logic programming in biomedical applications, focusing on the design and implementation of DNA logic programming and further exploring its advantages and challenges in frontier research areas.