Figure 8. Upper cases (A, B) represent merged fluorescent images on the
magnitude X1000. The red spots represent labeled hydrogel, and the
nucleus is stained with DAPI (blue). (A) and (B) represent cellular
uptake of hydrogel alone and in complex with CB8 correspondingly. Lower
cases (a, b) represent the correspondent phase-contrast images on the
magnitude X400 where arrows indicate the cytoplasm. The concentration of
each hydrogel was 0.18 mg/mL.
Supramolecular Effects on Cellular Internalization (Role of
Size, Charge, and Rigidity)
Overall, these data show that the modified ALG (drug carriers), by
covalently anchoring VG tags into the polysaccharides that are readily
non-covalently complexed to CB8 macromolecules have a distinct ability
to cross leukemia cancer cells (Figure 9), giving a more explicit
description of the cellular uptake mechanisms of supramolecular
hydrogels in cancer therapy.