Water returned to the atmosphere as evapotranspiration (ET) is approximately 1.6x greater than global river discharge and has wide-reaching impacts on groundwater and streamflow. In the U.S. Midwest, widespread land conversion from prairie to cropland has altered spatiotemporal patterns of ET, yet there is no consensus on the direction of change in ET or the mechanisms controlling changes. We aimed to harmonize findings about how land use change affects ET in the Midwest. We measured ET at three locations within the Long-Term Agroecosystem Research (LTAR) network along a latitudinal gradient with paired rainfed cropland and prairie sites at each location. At the northern locations, the Upper Mississippi River Basin (UMRB) and Kellogg Biological Station (KBS), the cropland has annual ET that is 84 and 29 mm/year higher, respectively, caused primarily by higher ET, likely from soil evaporation during springtime when agricultural fields are fallow. At the southern location, the Central Mississippi River Basin (CMRB), the prairie has 69 mm/year higher ET, primarily due to a longer growing season. To attribute differences in springtime ET to specific mechanisms, we examine the energy balance using the Two-Resistance Method (TRM). Results from the TRM demonstrate that higher surface conductance in croplands is the primary factor leading to higher springtime ET from croplands, relative to prairies. Results from this study provide critical insight into the impact of land use change on the hydrology of the U.S. Corn Belt by providing a mechanistic understanding of how land use change affects the water budget.