Primer Name | Primer Sequence 5’ 3’ |
EMSA primers | EMSA primers |
5’_pagR_EMSA (5’ 6-FAM label) | CGCAAATTTCCAGCGCCTGCG |
3’_pagR_EMSA (5’ HEX label) | TACACCGGCAAGTTGGGCTAC |
Deletion, Cloning, and RT-qPCR primers | |
5’_pagR_wanner
|
ATGTCGATACCCCGTAAACGGCGCAGTA CCGGTAAAGTGTAGGCTGGAGCTGCTTC |
3’_pagR_wanner
|
TTAGCAGCTTTCCCGCATACATAAACTG GAGGCGATCATATGAATATCCTCCTTAG |
5’_pagR_BspQI | NNGCTCTTCNTTCATGTCGATACCCCGTAAACG |
3’_pagR_BspQI | NNGCTCTTCNTTATTAGCAGCTTTCCCGCATAC |
5’_tktD_BspQI | NNGCTCTTCNTTCATGAACGTAACCGAAA |
3’_tktE_BspQI | NNGCTCTTCNTTATTAAAGCAGTGATTTCG |
5’_tktA_BspQI | NNGCTCTTCNTTCATGTCCTCACGTAAAGAGCTTG |
3’_tktA_BspQI | NNGCTCTTCNTTATTACAGCAGTGCTTTCGCTT |
5’_tktB_BspQI | NNGCTCTTCNTTCATGTCCCGTAAAGACCTTGC |
3’_tktB_BspQI | NNGCTCTTCNTTATTAGCCTTTGATGTTTAGCACC |
5’_tktD_wanner
|
TCACCCAACTGGCGCGCGATATTCGCGT CGCCACGCGTGTAGGCTGGAGCTGCTTC |
3’_tktE_wanner
|
TTTCGCGGCTTCTACAATCGCTTCGGCGG TGAGTCCCATATGAATATCCTCCTTAG |
5’_stm2343_qPCR | GTTCCGGCCTGGGTAGTAGTT |
3’_stm2343_qPCR | CCGATGAAAGATCGGAGTGT |
5’_tktD_qPCR | CGATGATATCGCCGGACTAC |
3’_tktD_qPCR | CCTCATTGAGCGTCTGCTTC |
5’_tktE_qPCR | CTATCGATCGCATGTTGGTG |
3’_tktE_qPCR | CTGCCCATAACGCTCTTTGA |
Operon PCR Primers | |
5’_operon PCR_1 | AGCGCCATAAAGAGCTGA |
3’_operon PCR_1 | CGATGATATCGCCGGACTAC |
5’_operon PCR_2 | TATTCAGCTCTTCGCGCG |
3’_operon PCR_2 | TCCTGCCAGTGCTGTTAC |
5’_operon PCR_3 | ACATACCCCCTAGCGG |
3’_operon PCR_3 | GTTCCGGCCTGGGTAGTAGTT |
5’_operon PCR_4 | CCGATGAAAGATCGGAGTGT |
3’_operon PCR_4 | TTGGGCCATATTATGTATTAGCG |
DNase 1 Footprinting Primers | |
5’_sequencing template | GGTACTGCGCCGTTTAC |
3’_sequencing template | GAACAATCGCCGTAAGGTATTC |
5’_digestion/sequencing (5’ 6-FAM label)
|
CGCAAATTTCCAGCGCCTGCG (same as 5’_pagR_EMSA) |
3’_digestion/sequencing (5’ HEX label) | TGTCGTTCTTTGCTTCGCATGAC |