Cell Culture
Human osteosarcoma cell line (U2OS; ATCC-HTB-96) were cultured at 37℃ in a humidified 5% CO2 atmosphere in DMEM supplemented with 10% FBS. U2OS WT+ GFP-MATK stables were additionally supplemented and cultured with 4 µg/mL puromycin. MMP-2 knockout U2OS cells were generated by CRISPR/Cas-9, as previously described (7).