FlowCam setup details for analysis with the 4x objective |
FlowCam setup
details for analysis with the 4x objective |
Flow cell sizes and types used, and objectives used for each flow cell |
300x1500 μm flow cell (FOV), 4x objective |
Calibration factor |
1.8130 |
Image acquisition mode |
Auto-image |
Flow rate |
0.9 mL min-1
|
Example of full context settings for 20180817_2205_001_NSP
|
Run:
Mode: AutoImage
Priming Method: machine prime
Flow Rate: 0.900 ml/min
Recalibrations: 0
Stop Reason: Sample Volume Processed
Sample Volume Aspirated: 9.9892 ml
Sample Volume Processed: 9.9544 ml
Fluid Volume Imaged: 6.9242 ml
Efficiency: 34.9%
Particle Count: 1542
Images:
Total: 2648
Used: 1166
Percentage Used: 44.03%
Particles Per Used Image: 1.32
Particles Per Image: 0.58
Particles Per Image: 0.58
Frame Rate: 3.99 fps
Intensity Mean: 168.49
Intensity Min: 167.68
Intensity Max: 169.85
Date/Time:
Start: 2019-09-10 11:04:32
End: 2019-09-10 11:16:10
Sampling Time: 00:11:03
Environment:
Software: VisualSpreadsheet 4.15.1
Magnification: 4X
Calibration Factor: 1.8130
GumStix Firmware: 23
Calibration Factor: 1.8130
SerialNo: 10446
Number of Processors: 8
Pump: C80 Syringe
Syringe Size: 5.00 ml
Capture Parameters:
Distance To Neighbor=50
Threshold Dark=15.0000
Threshold Light=18.0000
Close Holes=0
Capture Dark or Light Pixels=2
|