FlowCam setup details for analysis with the 4x objective FlowCam setup details for analysis with the 4x objective
Flow cell sizes and types used, and objectives used for each flow cell 300x1500 μm flow cell (FOV), 4x objective
Calibration factor 1.8130
Image acquisition mode Auto-image
Flow rate 0.9 mL min-1
Example of full context settings for 20180817_2205_001_NSP
Run: Mode: AutoImage Priming Method: machine prime Flow Rate: 0.900 ml/min Recalibrations: 0 Stop Reason: Sample Volume Processed Sample Volume Aspirated: 9.9892 ml Sample Volume Processed: 9.9544 ml Fluid Volume Imaged: 6.9242 ml Efficiency: 34.9% Particle Count: 1542 Images: Total: 2648 Used: 1166 Percentage Used: 44.03% Particles Per Used Image: 1.32 Particles Per Image: 0.58 Particles Per Image: 0.58 Frame Rate: 3.99 fps Intensity Mean: 168.49 Intensity Min: 167.68 Intensity Max: 169.85 Date/Time: Start: 2019-09-10 11:04:32 End: 2019-09-10 11:16:10 Sampling Time: 00:11:03 Environment: Software: VisualSpreadsheet 4.15.1 Magnification: 4X Calibration Factor: 1.8130 GumStix Firmware: 23 Calibration Factor: 1.8130 SerialNo: 10446 Number of Processors: 8 Pump: C80 Syringe Syringe Size: 5.00 ml Capture Parameters: Distance To Neighbor=50 Threshold Dark=15.0000 Threshold Light=18.0000 Close Holes=0 Capture Dark or Light Pixels=2