FlowCam setup details for analysis with the 20x objective |
FlowCam
setup details for analysis with the 20x objective |
Flow cell sizes and types used, and objectives used for each flow cell |
50x300 μm flow cell (FOV), 20x objective |
Calibration factor |
0.3380 |
Image acquisition mode |
Auto-image |
Flow rate |
0.03 mL min-1
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Example of full context settings for 20180816_0356_001_NSP
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Run:
Mode: AutoImage
Priming Method: machine prime
Flow Rate: 0.030 ml/min
Recalibrations: 1
Stop Reason: Sample Volume Processed
Sample Volume Aspirated: 1.0000 ml
Sample Volume Processed: 0.9990 ml
Fluid Volume Imaged: 0.3388 ml
Efficiency: 33.9%
Particle Count: 1239
Images:
Total: 38201
Used: 1219
Percentage Used: 3.19%
Particles Per Used Image: 1.02
Particles Per Image: 0.03
Particles Per Image: 0.03
Frame Rate: 19.57 fps
Intensity Mean: 177.09
Intensity Min: 174.78
Intensity Max: 177.72
Date/Time:
Start: 2019-08-22 10:48:26
End: 2019-08-22 11:21:52
Sampling Time: 00:32:31
Environment:
Software: VisualSpreadsheet 4.15.1
Magnification: 20X
Calibration Factor: 0.3380
GumStix Firmware: 23
Calibration Factor: 0.3380
SerialNo: 10446
Number of Processors: 8
Pump: C80 Syringe
Syringe Size: 0.50 ml
Capture Parameters:
Distance To Neighbor=30
Threshold Dark=15.0000
Threshold Light=18.0000
Close Holes=0
Capture Dark or Light Pixels=2
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