High-relief glacial valleys shape the modern topography of the Southern Patagonian Andes, but their formation remains poorly understood. Two Miocene plutonic complexes in the Andean retroarc, the Fitz Roy (49°S) and Torres del Paine (51°S) massifs, were emplaced between 16.9–16.4 Ma and 12.6–12.4 Ma, respectively. Subduction of oceanic ridge segments initiated ca. 16 Ma at 54°S, leading to northward opening of a slab window with associated mantle upwelling. The onset of major glaciations caused drastic topographic changes since ca. 7 Ma. To constrain the respective contributions of tectonic-mantle dynamics and fluvio-glacial erosion to rock exhumation and landscape evolution, we perform inverse thermal modeling of a new dataset of zircon and apatite (U-Th)/He from the two massifs, complemented by apatite ⁴He/³He data for Torres del Paine. Our results show rapid rock exhumation recorded only in the Fitz Roy massif between 10 and 8 Ma, which we ascribe to local mantle upwelling forcing surface uplift and intensified erosion around 49°S. Both massifs record a pulse of rock exhumation between 7 and 4 Ma, which we interpret as enhanced erosion during the beginning of Patagonian glaciations. After a period of erosional and tectonic quiescence in the Pliocene, increased rock exhumation since 3-2 Ma is interpreted as the result of alpine glacial valley carving promoted by reinforced glacial-interglacial cycles. This study highlights that glacial erosion was the main driver to rock exhumation in the Patagonian retroarc since 7 Ma, but that mantle upwelling might be a driving force to rock exhumation as well.