Simulated ILDF System
The simulated ILDF system consists of the same or similar equipment used
in the batch process. The equipment was arranged in a manner supporting
multiple independent concentration/buffer exchange passes with
opportunity for sampling and dilution with buffer in between each pass
(Figure 1D). The hollow fiber cartridge was not replaced in between
passes. A new hollow fiber was used for each bulk run on the simulated
ILDF system.
The starting solutions and liposome mixture as described previously were
used as a starting material. The buffer solution was used to pre-dilute
the liposome mixture as described in the Results Section.