Purification of the pooled E. coli K-12 ORFeome plasmid library
A previously described E. coli ORFeome plasmid library (Rajagopala et al. , 2010) was received as a stab stock in a 96-well plate format (National BioResource Project, Japan). The clones were grown in LB medium supplemented with 50 µg/mL Zeocin for 24 hours at 37°C (Velp Scientifica FTC 90I). The cell cultures were pooled, and the plasmids were purified using a NucleoBond Xtra Midi kit (Macherey-Nagel).