Fig. 6. Ribosomal fractions of ΔybeX contain distinct 16S rRNA and 17S pre-rRNA fragments. (A ) Sucrose gradient profiles of WT andΔybeX . The strains were grown in LB medium at 37°C for 2 hours after the OD600 reached 0.3. 10-30% sucrose gradients were used for sedimentation. The gradient profiles are representative of four independent experiments. (B -F ) Northern blot hybridization of the same membrane using different Cy5-labelled oligonucleotides (see Table S3 and Fig. 5A). Truncated ribosomal RNA species are annotated as “trunc.”. The Cy5-labelled fluorescence oligonucleotides are 16S 3‘-end, 16S (16S EUB), 17S 3‘-end, 17S 5‘-end, 23S (23S h25) specific, and each probe indicated under panels B-F.