Fig.
4. The ΔybeX-dependent antibiotic sensitivity and outgrowth delay
depend on the growth phase of the inoculum. (A ) Drops of the
stationary phase and exponential phase cultures of the Keio wild-type
(WT), ΔybeX , ΔybeZ , and ΔybeY strains were spotted
on LB-agar plates. The cells were cultured in LB medium, and the LB agar
plates were incubated at 37°C, except for the 42°C plates.
(B-C ) Stationary and exponential phase cells of wild-type,
ΔybeY , ΔybeZ , and ΔybeX strains were inoculated
into liquid LB medium, and the growth was monitored by measuring
OD600 at 37°C using a 96-well plate reader. The growth
curves, summarizing three independent experiments, are shown as fitted
logistic curves. Shaded areas represent the 95% CI-s.