Fig.
1. Growth phenotypes of ΔybeX strain and compensation with
single-copy plasmid. (A ) The E. coli ybeZYX-lnt operon
chromosomal organization with sigma factors (σ32 and
σ24) and promoters (ybeZp and the predicted lntp4).
(B ) Dot spot assay of wild-type (WT) and ΔybeX strains,
along with the strains harbouring the TransBac library plasmid backbone
(WT/pEmpty or ΔybeX /pEmpty), and the ΔybeX strain with the
YbeZ, YbeY, or YbeX-expressing single-copy TransBac library plasmid. LB
agar plates were incubated at 37°C or 42°C. (C-D ) The
stationary phase outgrowth of the wild type and the ΔybeX strains
in liquid LB medium. Panel C shows the OD600 signal, and
panel D shows the Alamar Blue fluorescence reading. Both signals are
normalized to one. Individual measurements from independent experiments,
each presented as the mean of three technical replicates, are shown as
dots. Curves are modelled with splines, and 95% credible intervals are
shown as shaded areas (see Materials and Methods for details). pybeX,
pybeZ and pybeY denote the YbeX, YbeZ and YbeY expressing plasmid.