Histopathology
CIA mice were sacrificed, and the joints were removed. The specimens
were then fixed in 10% phosphate-buffered formalin and decalcified for
30 days in 10% EDTA or 5% formic acid. After decalcification, the
joints were embedded in paraffin and histological examination was
performed using hematoxylin and eosin (HE) staining (Servicebio, Wuhan,
China).