5. Conclusion

In this study, 616 samples of Tetraodontiformes were identified by using morphological characteristics and DNA barcoding technology. It revealed that DNA barcoding can be effectively used in the identification of Tetraodontiformes.
Moreover, it is suggested that DNA barcoding can provide molecular evidence for clarifying the problem of species synonyms and confirming valid species names. It provided molecular evidence for clarifying the valid species names of L. Spadiceus and T. rubripes .
In addition, this study reconfirms that there are some incorrect sequences in both NCBI and BOLD databases. It is suggested that synonyms and the unreliable data are also the reasons for the confusion of taxonomic identification of Tetraodontiformes. We suggest that when using DNA barcode technology to identify species, the sequence in the database needs to be strictly screened. When the sample has complete morphological characteristics, the final identification results of species should be determined by combining the morphological characteristics.