Materials and methods
2.1 Sample collection
Since 2011, our team has collected Tetraodontiformes specimens from the
coastal waters of China. In the past 11 years, a total of 616 specimens
of Tetraodontiformes have been collected (Table 1). After rough
classification according to morphologicalcharacteristics, they were
preserved in 95% ethanol for subsequent DNA barcode identification. All
voucher specimens are deposited in the National Marine Fishery
Biological Germplasm Resource Bank.