5. Conclusion
In this study, 616 samples of Tetraodontiformes were identified by using
morphological characteristics and DNA barcoding technology. It revealed
that DNA barcoding can be effectively used in the identification of
Tetraodontiformes.
Moreover, it is suggested that DNA barcoding can provide molecular
evidence for clarifying the problem of species synonyms and confirming
valid species names. It provided molecular evidence for clarifying the
valid species names of L. Spadiceus and T. rubripes .
In addition, this study reconfirms that there are some incorrect
sequences in both NCBI and BOLD databases. It is suggested that synonyms
and the unreliable data are also the reasons for the confusion of
taxonomic identification of Tetraodontiformes. We suggest that when
using DNA barcode technology to identify species, the sequence in the
database needs to be strictly screened. When the sample has complete
morphological characteristics, the final identification results of
species should be determined by combining the morphological
characteristics.