Enzyme |
The concentration of DMSO (v/v, %) |
Substrate load
(mM) |
Reaction time (h) |
Yield (%) |
Optical purity of product
(e.e.) |
WT |
25 |
3 |
10 |
88.3 ± 3.1 |
> 99.5%
R
|
|
25 |
5 |
10 |
17.3 ± 1.0 |
> 99.5%
R
|
|
25 |
10 |
10 |
10.9 ± 0.3 |
> 99.5%
R
|
|
25 |
15 |
10 |
4.3 ± 0.7 |
> 99.5%
R
|
|
25 |
20 |
10 |
2.3 ± 0.1 |
> 99.5%
R
|
M3 |
25 |
3 |
10 |
94.2 ± 4.2 |
> 99.5%
R
|
|
25 |
5 |
10 |
69.5 ± 0.9 |
> 99.5%
R
|
|
25 |
10 |
10 |
38.9 ± 1.2 |
> 99.5%
R
|
|
25 |
15 |
10 |
15.3 ± 0.8 |
> 99.5%
R
|
|
25 |
20 |
10 |
8.7 ± 0.3 |
> 99.5%
R
|