Figure 9. Time course of asymmetric synthesis of (R )-NEA on 20-mL scale by using recombinant E.coli whole cells expressing WT and M3. Reaction was performed with 3 mM (A), 5 mM (B), 10 mM (C), 15 mM (D), and 20 mM (E) 1-acetylnaphthalene and a corresponding equivalent 1-(R )-PEA in 50 mM, pH 8.0 PBS buffer with DMSO (25%,v/v ) at 30°C.