Figure 5
3.7 Clarifying the mechanism of organic solvents stability enhancement
In order to investigate the effect of solvent on enzyme surface, the
parameter of Solvent Accessible Surface Area (SASA) was calculated via
Discovery Studio Client 2019. SASA is the surface area of biomolecules
that can be contacted by solvent, and a SASA value represents the
aggregation-related properties of enzymes. A smaller SASA value implies
a tighter packed structure of enzymes.[34] As
shown in Figure 6, the average SASA value of WT was 22244.4
Å2, and, the value of M3 reduced to 22163.2
Å2. The decreased SASA value of M3 suggested that the
combinatorial mutation of triple mutants resulted in a more tightly
packed structure to enhance the organic solvents stability.