Figure 5
3.7 Clarifying the mechanism of organic solvents stability enhancement
In order to investigate the effect of solvent on enzyme surface, the parameter of Solvent Accessible Surface Area (SASA) was calculated via Discovery Studio Client 2019. SASA is the surface area of biomolecules that can be contacted by solvent, and a SASA value represents the aggregation-related properties of enzymes. A smaller SASA value implies a tighter packed structure of enzymes.[34] As shown in Figure 6, the average SASA value of WT was 22244.4 Å2, and, the value of M3 reduced to 22163.2 Å2. The decreased SASA value of M3 suggested that the combinatorial mutation of triple mutants resulted in a more tightly packed structure to enhance the organic solvents stability.