Figure 2. The structural model of At ATA (PDB ID: 4CE5) and the targeting regions for epPCR. The three loop regions and the corresponding sequences at the surface of enzyme were colored in red.
Figure 3. An overview of the effect of each mutant on At ATA-WT relative activity (A). All the mutants were incubated in 80% (v/v ) DMSO for 30 min at 30 oC to assay the residual activity. The residual activity of At ATA-WT was defined as 100%. The specific activity of WT and mutants (B). The purified enzymes were incubated or no incubated in 25% DMSO for 15 min at 30°C to assay the specific activity.
Figure 4. Residual activities of WT and its variants toward 1-acetylnaphthalene. The purified enzymes were incubated in 15%, 25%, 35% and 45% DMSO for 15 min at 30°C. Then, the residual activity was assayed in the mixtures containing 0.5 mg/mL purified enzymes, 5 mM 1-(R )-PEA ,5 mM 1-acetylnaphthalene, 0.1 mM PLP and 25% (v/v ) DMSO at 30°C, 600 rpm for 30 min.