Supplementary Figure Legends
Figure S1. (A) Serum IgE levels in naïve and T. muris(Tm)-infected mice measured by ELISA. Flow cytometric analysis for mast
cells (B) (CD45+Lineage
(Lin)−FcεRIα+IgE+CD49b+/-ckit+)
in naïve and Tm-infected mice showing percentage of
Lin- in (C) cecum and (D) spleen. (E) MLN basophil
gating (CD45+Lineage
(Lin)−FcεRIα+IgE+CD49b+ckit-).
Basophil percentage of (F) Lin- and (G)
CD45+ MLN cells. Data from all combined experiments
are presented as the mean ± SEM. Statistical analysis was performed
using a linear fixed-effect model with pairwise comparison; P ≤ 0.05,
**P ≤ 0.01, *** P ≤ 0.001. n=2-3 for each time point per experiment; 3
independent experiments.
Figure S2. Flow cytometric analysis of basophils in the spleen
in naïve and T. muris (Tm)-infected mice, displaying (A)
representative plots for Notch2 staining, (B) percent
Notch2+ basophils, and (C) Notch2 geometric mean
fluorescence intensity (gMFI) on spleen basophils. Data from all
combined experiments are presented as the mean ± SEM. Statistical
analysis was done using a linear fixed-effect model with pairwise
comparison; ***P ≤ 0.001. n=2-3 for each time point per experiment; 3
independent experiments.
Figure S3. Real time PCR analysis of proximal colon homogenate
from naïve and T. muris (Tm)-infected mice, quantifying (A)Il4 and (B) Il13 (normalized to Actb ). Data from
all combined experiments are presented as the mean ± SEM. Statistical
analysis was done using a linear fixed-effect model with pairwise
comparison; *P ≤ 0.05. n=2-3 for each time point per experiment; 3
independent experiments.
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