Experimental Mice and in vivo Treatments
Female AKR/J and C57BL/6 mice at 8-10 weeks old were purchased from The
Jackson Laboratory. The animals were housed in specific pathogen free
conditions at the Cornell University College of Veterinary Medicine
Baker Institute for Animal Health or the University of Washington South
Lake Union 3.1 animal facility. Animals were handled according to
protocols approved by the Cornell University and University of
Washington Institutional Animal Care and Use Committees. For IFN-γ
neutralization experiments, AKR/J mice were treated with 1 mg of
anti-IFN-γ (XMG1.2) or rat IgG1 (Isotype control) at Day -1, +3, +7, and
+11. Day 0 served as the day of infection with 200 T. muris eggs.