Figure legends:
Figure 1. ZnO nanoparticles characetrixation. FESEM image (A)
and XRD pattern (B) of ZnO nanoparticles.
Figure 2. Chitosan hydrogel characterization. FTIR spectrums of
chitosan polymer (A) and chitoan hudrogel (B). FESEM image of chitosan
hydrogel (C).
Figure 3. Swelling ratio
of different hydrogel formulations in PBS and citarte buffer mediums.
Figure 4. A) Drug
release profiles of different hydrogel formulations at pH 7.4 in the
presence and absence of ZnO nanoparticles. B) Drug release profiles of
hydrogel forulation with 2 mg genipin and ZnO nanoparticles at different
pHs 7.4 and 5.8. C) The release profile of ZnO nanoparticles from the
different hydrogel formulations in two different pH 7.4 and pH 5.8.
Figure 5 . Inhibition
zone diameters (mm) of S. aureus (green) and P.aeruginosa(blue) grown in the presence of vancomycin, chitosan hydrogel (CH),
chitosan hydrogel contained ZnO NPs (CNH) and chitosan hydrogel loaded
by vancomycin and ZnO NPs (CNH loaded vancomycin). Data are represented
as mean ± SD (n=3).
** Denotes significant
differences with p<0.0002 and
*** Denotes significant differences with p<0.0001.
Figure 6 . Bacterial number of S. aureus (green) andP.aeruginosa (blue) after treatment with chitosan hydrogel (CH),
chitosan hydrogel contained ZnO NPs (CNH) and chitosan hydrogel loaded
by vancomycin and ZnO NPs (CNH loaded vancomycin). Data are represented
as mean ± SD (n=3)
* Denotes significant differences with p<0.05 and
*** Denotes significant differences with p<0.0001