Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies. Table 1. Characteristics of the enrolled studies.
Study Year Country/Number of patients Anti-PD-L1 antibody Definition of PD-L1 expression in stained cells Assessment for IHC stained cells Anatomic location Clinicopathological variables assessment Prognostic variables assessment
Yoshida et al. 2018 Japan/135 28-8 Abcam TC:M Semiquantitative evaluation(≥50%) Only tongue Y Y
Adamski et al.
2021
Poland/109
E1L3N,Cell Signaling
TC:M TILs:M&C
Percentage of positive cells (TC:>10%; TILs:>20%)
Tongue,floor of the oral cavity,others.
Y
Y
Hirai et al. 2017 Japan/24 Abcam TC:M Percentage of positive cells(>10%) NE Y N
Cho et al. 2011 Korea/45 Abcam(clone ab82059) TC:M &C H-score(ranging 0-5,≥3) Tongue,floor of mouth, buccal mucosa, retromolar trigone, hard palate. Y Y
Lenouve et al. 2020 Spain/55 22C3 clone,Dako TC:M Tumour proportion score (TPS)≥5% NE Y Y
Troeltzsch et al. 2016 Germany/88 E1L3N, Cell signaling TC: NS Semiquantitative evaluation(≥5%) Tongue,alveolar process maxilla and hard palate,soft palate, floor of the mouth, alveolar process mandible. Y N
Quan et al. 2020 China/83 Clone E1L3N, CST IC: NS Density of cells(>10%) NE Y Y
Togo et al. 2020 Japan/59 28-8 Abcam IC:M Semiquantitative evaluation(≥10%) Tongue,Gingiva,Buccal mucosa,Oral floor,Lip. Y Y
Wirsing et al. 2017 Norway/75 Clone SP263, Ventana TC: M&C Percentage of positive cells(>10%) NE Y Y
Tojyo et al.
2019
Japan/49
790-4905 rabbit monoclonal, Ventana
TC:M TILs:M
Percentage of positive cells (TC:≥5%; TILs:≥1%)
NE
Y
Y
Geum et al. 2022 Korea/81 Abcam TC:N&M&C H-score(≥ mean value) maxilla,mandible,floor of mouth,Buccal mucosa +etc. Y Y
Lin et al. 2015 Taiwan, China/305 GTX104763 TC:M&C Intensity of staining (ranging 0-3,≥2) NE Y Y
Akisada et al. 2020 Japan/121 E1L3N,Cell Signaling TC:NS Percentage of positive TCs(≥50%) Only tongue Y Y
Pena-Cardelles et al. 2022 Spain/65 Clone 22C3, Dako TC:M CPS≥1,TPS≥5%/10% Tongue,mouth. Y Y
Miranda-Galvis et al. 2021 Brazil/123 Abcam(clone 73-10, ab228415) TC, IC: M TPS≥1%/5%/10% NE Y Y
Kikuchi et al. 2019 Japan/103 Clone 28-8; Abcam TC:M CPS≥1 NE N Y
Wang et al. 2019 China/36 Abcam, ab213524 TC:M H-score(≥4) NE N Y
de Vicente et al. 2018 Spain/125 Clone E1L3N, clone 22C3, Dako TC:NS Percentage of positive TCs(≥10%) Tongue,floor of the mouth,gum,buccal,retromolar,palate. Y Y
Kouketsu et al. 2017 Japan/106 Clone SP142,Ventana TC:M&C H-score(≥1) Tongue,Upper gingiva, Lower gingiva, Hard palate,Buccal mucosa, Floor of mouth,Lower lip. Y Y
Straub et al. 2016 Germany/80 Clone E1L3N TC:M Percentage of positive cells (≥5%) NE Y Y
Chen T-C et al. 2015 Taiwan, China/218 Proteintech Group TC:M Percentage of positive cells (≥5%) NE N Y
Takamaru et al. 2022 Japan/169 Clone SP142,Ventana TC:M Percentage of positive TCs(>1%) NE Y Y
Klein et al. 2021 Germany/58 22C3 clone,Dako TC:M Intensity of staining (ranging 0-3,≥2) lip cancer Y Y
Satgunaseelan et al. 2016 Australia/217 E1L3N-XP-Rb mAb TC: M Percentage of positive cells (≥5%) Tongue,floor of mouth, gingiva,buccal. Y Y
Takahashi et al. 2019 Japan/77 E1L3N, Cell signaling TC:M Semiquantitative evaluation(≥10%) NE Y Y
de Sa et al. 2021 Brazil/48 NS TC:M Intensity of staining(>0%) Only tongue N Y
Wilms et al. 2020 Sweden/101 Clone SP263, Ventana TC,IC: M&C H-score(ranging0-18,≥6) Only tongue Y Y
Huang et al. 2020 China/152 Abcam,ab52587 TC,IC: M&C H-score(≥2) Only tongue Y Y
Hanna et al. 2017 USA/81 Clone 9A11 TC:M&C Percentage of positive cells (≥10%) NE N Y
Maruse et al. 2018 Japan/97 Clone E1L3N, Cell Signaling TC:M&C Percentage of positive cells (≥5%) NE N Y
Ahn et al. 2017 Korea/68 Clone ab153991, Abcam TC:M&C Semiquantitative evaluation(≥10%) NE Y Y
Balermpas et al. 2017 Germany/41 Clone E1L3N TC:NS Percentage of positive cells(>5%) NE N Y
Foy et al. 2017 France/44 Clones SP142, 28.8 TC:NS Percentage of positivecells(≥1,≥5,and≥10%) NE Y N
Kogashiwa et al. 2017 Japan/84 Clones SP142 TC:M&C Percentage of positive cells(>5%) NE Y Y
Mattox et al. 2017 USA/53 Clone 5H1 TC: M Percentage of positive TCs and/or TILs (> 1%) Only tongue Y Y
Moratin et al. 2019 Germany/175 Cell signaling TC:NS H-score(≥4) NE Y Y
Naruse et al. 2019 Japan/121 Abcam(clone ab156361) TC:C&N Percentage of positive TCs(>5%) Only tongue Y Y
Oliveira-Costa et al. 2015 Brazil/96 Abcam(clone ab28753) TC:M, C Percentage of positive cells (>5%) NE Y Y
Schneider et al. 2018 Austria/36 Clone 5H1 TC:M Percentage of positive cells (>5%) Only tongue N Y
Tsai et al. 2019 Taiwan, China/173 NS NS H-score NE N Y
Udeabor et al. 2018 Nigeria/20 28-8 Abcam TC:NS H-score(≥0) NE Y N
Manikhas et al. 2018 Russia/82 clone BCDdx1020 by ICH TC:NS Percentage of positive cells (TC:≥1%;≥5%) NE N Y
Ahmadi et al. 2019 Australia/255 E1L3N-XP-Rb mAb TC:M Percentage of positive TCs (≥1%) NE Y Y
Subramaniam et al. 2021 India/64 NS TC,TILs:M&C Intensity of staining (ranging 0-3,≥1) Tongue and buccal mucosa. Y Y
Chen X-J et al. 2019 China/41 clone SP142, abcam TC:C Percentage of positive cells (≥5%) Only tongue N Y
Zhao et al. 2019 China/46 ab205921,abcam TC:M&C Percentage of positive TCs (≥50%) Only tongue Y N
Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score. Abbreviations: TC: tumor cell; IC: immune cell; IHC: immunohistochemistry; M: membranous; C: cytoplasmic; N:nuclei; NS:not stated; NE: not examined. H:defined as staining intensity × the percentage of staining cells; CPS: combined positive score; TPS: tumour proportion score.