Figure legends
FIGURE 1 General regulatory mechanism of Rap-Phr pairs. (i) the
Rap protein is produced and, (ii) carries its regulatory role
intracellularly; meanwhile, (iii) pre-Phr proteins are produced, (iv)
processed and exported out of the cell as Phr peptides; (v) upon
reaching threshold concentrations, (vi) mature Phr peptides can be
imported into the cell via the Opp permease and (vii) inhibit its
cognate Rap protein. Rap proteins are visualized using the RapF crystal
structure (PDB doi: 10.2210/pdb4I9E/pdb), while prePhr is depicted using
the structure based on AlphaFold as displayed in SubtiWiki . The figure
was prepared on BioRender.com.
FIGURE 2 Known cellular functions regulated by Rap phosphatases
in B. subtilis . The centeral protein structure shows the
conserved architecture of Rap phosphatases (RapF crystal structure, PDB
doi: 10.2210/pdb4I9E/pdb). The biofilm colony images of wild type (WT)
and ΔrapP mutant are taken from Nordgaard et al. (2021),
scale bar represents 5 mm. The figure was prepared on BioRender.com.