2.4 Protocol 2: Assorted information of specimens with or
without voucher exuviae
Culturing and morphological data. This method is clearly
distinguished from Protocol 1 by retaining notably fewer data layers
than Protocol 1. For example, while the WS-specimens are represented by
all data layers, except for live images of the sequenced specimens, the
AZ-material is exclusively represented by exuvial vouchers photographed
in LM. Although admittedly artificial, we decided to lump all cases with
varying degrees of morphological information, but no live imaging, into
one category. Protocol 2 was applied to the three live-imaged specimens
of H. demodex from Green Island (TA) in Olesen et al., (2022),
the specimens of H. itoi from the White Sea (WS; for which all
data layers except live-images of single specimens exist), and the
Azores specimens (AZ; for which only y-naupliar exuviae are available).
2.4.1 DNA extraction. We tested three extraction procedures: that
used exclusively for Protocol 1, the DNeasy™ kit (as described in detail
above and in the Supplementary Material) following the manufacturer’s
specifications, and the GeneReleaser™ kit (as described in detail
above).