In this study, we offer significant improvement over previous results that identified the Matuyama-Brunhes magnetic reversal in cave sediments from the Czech Republic in Central Europe. We collected discrete samples from the sedimentary profile in Za Hajovnou cave located in the eastern part of the Czech Republic. The rock magnetism measurements indicated that the magnetic carrier of most of the samples is maghemite. Characteristic remanent magnetization (ChRM) directions and related virtual geomagnetic pole (VGP) paths indicated that the Matuyama-Brunhes transition boundary was within 5.7 cm of the sediment, located in the upper part of the sampled sedimentary section. This result showed a new, more detailed behavior of the polarity transition from that of the central European location. The migration of the paleopole between eastern Africa and western North America was established as a significant marker for the central European paleomagnetic record in terms of global magnetic data. The transition duration was 8.1±0.2 kyr, and the precursor of the reversal occurred 4±0.2 kyr before the transition. In addition, we estimated the sedimentation rate of the studied section (~35 cm) in the cave as 0.7±0.2 cm/kyr.