Abstract
Effective therapies to reduce ischemia/reperfusion and
hypoxia/reoxygenation injury are currently lacking. Borneol has a clear
protective effect on ischemia-reperfusion injury, and its protective
effect on myocardial hypoxia/reoxygenation and its mechanism have
remained to be elucidated. This study investigated the protective
effects of borneol on H9C2 cardiomyocytes during hypoxia/reoxygenation
injury and to explore the underlying molecular mechanisms. H9C2 rat
cardiomyocytes were cultured, the MIRI model was established by hypoxia
for 5h and reoxygenation for 24h. The cells were divided into control
group, hypoxia/reoxygenation group (H/R group), (low-, medium-, high-)
dose borneol group. Borneol administration groups were pre-treated for
12h with different doses of borneol prior to hypoxia/reoxygenation, 24h
after reoxygenation, the cells or culture medium were collected for
testing. The results showed that borneol has no cytotoxicity and can
enhance the viability of cardiomyocytes, significantly increased the SOD
content, significantly decreased LDH leakage in cell culture medium, and
reduced evidently the apoptosis rate, down-regulated the expression of
Bax and Caspase-3. In conclusion, this study demonstrated that borneol
has a definite protective effect on hypoxia/reoxygenation-induced injury
of rat cardiomyocytes, and its protective mechanism can be related to
the inhibition of apoptosis by reducing the expression of Bax and
Caspase-3.