The dispersal of dissolved iron (DFe) from hydrothermal vents is poorly constrained. Combining field observations and a hierarchy of models, we show that the dispersal of DFe from the Trans-Atlantic-Geotraverse vent site occurs predominantly in the colloidal phase and is controlled by multiple physical processes. Enhanced mixing near the seafloor and transport through fracture zones at fine-scales interacts with the wider ocean circulation to drive predominant westward DFe dispersal away from the Mid-Atlantic ridge at the 100km scale. In contrast, diapycnal mixing predominantly drives northward DFe transport within the ridge axial valley. The observed DFe dispersal is not reproduced by the coarse resolution ocean models typically used to assess ocean iron cycling due to their omission of local topography and mixing. Unless biogeochemical models include high-resolution nested grids, they will inaccurately represent DFe dispersal from axial valley ridge systems, which make up half of the global ocean ridge crest.