Purpose: Introducing tricuspid annular systolic excursion (TAPSE)-slope and assessing inter-observer variability of its timing measurements Methods: We performed comprehensive Doppler echocardiographic examinations on 84 healthy subjects (mean age: 36.3 years, range: 30-50 years, 51 women, 33 men). In color-coded M-mode tracing of right ventricle’s free wall, peak systolic excursion was defined. TAPSE and time from initial QRS to peak systole in color-coded M-mode were measured, and TAPSE-slope was calculated based on this formula: TAPSE slope=TAPSE/TAPSE-t. For the evaluation of inter-observer variability, two other cardiologists measured TAPSE-t in offline mode separately. Results: The average (SD) of the “TAPSE-slope” was 7.0(1.0) cm/sec, the average (SD) TAPSE was 2.42(0.3) cm, and an average TAPSE-t of 343(27.6) msec. A significant difference was seen in TAPSE-slope between men and women (P value=0.001). No significant difference was detected in TAPSE-slope in people younger than 40 years old compared with participants older than 40 years. Intra-class correlation coefficient value of the TAPSE-t measurement was 0.886, showing excellent agreement between three different raters. Conclusion: TAPSE-slope may have added value compared to TAPSE alone for the assessment of right ventricular systolic function.