3Phenokey, s’Gravenzande, Netherlands
ORCiD: [0000-0001-7525-3705]
Keywords: phenotyping, spike, seed, seedling, germination,
X-ray, automation.
During the last years, X-ray technology has been applied for the
non-destructive visualization of optical inaccessible plant structures.
With X-ray computed tomography (CT) the 3D volume information of objects
can be reconstructed using X-ray projections of the object from
different points of view. This enables the non-destructive analysis of
hidden structures like root systems or the analysis of internal seed
organs. Thus, seeds can be phenotyped in as single seed, bunch of seeds,
spikes and during germination. With automation, this high throughput CT
analysis can be done. All plant organs can be automatically segmented
and measured in 3D, currently 50 seedlings in 2 minutes, or 200 seeds
within 5 minutes. Additionally, tracing of individual seedlings over
time across the developmental process is possible, without disrupting
the germination containers. The process can be run in fully-automated
and is industrially validated for multiple years.
In this presentation, we will demonstrate the range of applications for
X-ray based seed analysis. This ranges from a high resolution single
seed approach over batches of seeds to the germination testing. The
output of the measurements are segmented seed or plant organs for each
individual seed and plant. This process enables a quantitative,
objective and reproducible assessment of morphological seed and seedling
traits in 4D, which can substitute visual germination and vigor testing.
We will present traits such as seed quality, seedling development,
degree of abnormalities, germination capacity and vigor of seed lots of
different crop types.
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