Records from systematic studies
We searched for roadkill survey conducted in Ecuador and described in peer-reviewed publications and theses. This search was performed in English and Spanish (official language of Ecuador) using the keywords “roadkill Ecuador”, “wildlife mortality Ecuador”, “amphibians roadkills Ecuador”, “birds roadkills Ecuador”, “mammals roadkills Ecuador”, “reptiles roadkills Ecuador”, “snakes roadkills Ecuador”, “frogs roadkills Ecuador”, “atropellamiento fauna Ecuador”, “atropellamiento anfibios Ecuador”, “atropellamiento aves Ecuador”, “atropellamiento mamíferos Ecuador”, “atropellamiento reptiles Ecuador”, “atropellamiento serpientes Ecuador”, “atropellamiento sapos Ecuador”, “atropellamiento ranas Ecuador”. When a study was located, but the roadkill records were not available, we contacted authors asking to provide their data.
From each identified study we collected: taxonomic information of roadkilled organisms, number of roadkills per species or lowest identified taxonomic group, length of road surveyed, survey method (car, motorcycle, bicycle, or walking), time interval between surveys, total sampling period, and geographic coordinates of each roadkill record if available. As original records could include synonyms or obsolete species names, we additionally provide taxonomic information standardized using the IUCN nomenclature. We removed any records of domestic and farm animals. For each study we then calculated roadkill rates per species (only specimens identified to species were considered) by dividing the total number of records by the total length in kilometers of the surveyed road(s) and the total sampling period (days) (number of days between the first day until the last day of fieldwork). This value was converted to number of individuals per km per year (ind./km/year) multiplying by 365. Standardized rates allow comparison of road mortality among species and areas where systematic studies have been conducted.