Figure 5 Effects of auxotrophic genes from nucleotide module on cell growth and FFA production. Control: prototrophic strain;ura3 Δ: strain ScYan1 with single damage of gene URA3 ;ade2 Δ: strain ScYan15 with single deletion of gene ADE15 . Growth curve of strain ura3 Δ and ade2 Δ were measured by adding exogenous histidine of 20, 60 and 100 mg/L, respectively (A and B). Fatty acid titers at fermentation endpoint (48 h) of ura3 Δ and ade2 Δ were measured (C and D). All data was represented as the mean ± s.d. of three clone samples.