Figure 3 Effects of auxotrophic genes from succinyl-CoA module on cell growth and FFA production. Control: prototrophic strain;met15 Δ: strain ScYan10 with single deletion of gene MET15 . Growth curve (A) and fatty acid titers (B) at fermentation endpoint (48 h) of met15 Δ were measured by adding exogenous methionine of 100, 500 and 1,000 mg/L, respectively. All data was represented as the mean ± s.d. of three clone samples.