Lab Analyses
The lab analyses consisted of three related but separate protocols (Figure 1). The first was to assess the bacterial composition of the fish gut and rearing water microbiomes using 16S rRNA metabarcoding. The second was to determine the whole transcriptome response to treatment by RNA-Seq of gut tissue from offspring from a single family. The third analysis was designed to better characterize the transcriptional profile response to the treatments using nanofluidic array qPCR analysis of 50 gene loci selected using the RNA-Seq analysis.