Functional verification of PIM1
To validate the function of PIM1, 4 male tree sparrows from LJX were dosed daily by oral gavage at 100 mg/kg of SGI-1776, an experimental PIM1 kinase small molecule inhibitor, and the same numbers of LJX males were used as control group. After 1 week’s treatment, we analyzed the sperm traits of two groups. However, we find there is no observed sperm traits variation between two groups. The sperm length and velocity of PIM1 inhibitor treatment group show no difference with those of the control group and the natural LJX population (Figure 3C). We then use the transcriptome data to quantify PIM1 expression level, and we find that the PIM1 expression level of treatment group is similar to control group.