Residue Interaction network analysis
The protein conformations were visualized in Chimera [81] and
interaction networks were obtained using Structure Viz [82], a
Cytoscape [83] plugin, having contacts with default parameters set
as criteria for building the network which was visualized using
Cytoscape. Topological analysis like betweenness centrality (BC),
closeness centrality (CC) were calculated using CytoNCA [84],
another cytoscape plugin, each giving inputs regarding the functionally
relevant residues. Also Z-score for BC & CC were calculated using the
formula, z = (x-μ)/ σ, where x is the actual value, μ is the mean of the
dataset, and σ is the standard deviation of the dataset.