Systems
The 41 amino acid long tau sequence from residue number 340 to 380, along with coordinates were extracted from protein with PDB-ID 6TJX, chain A [69]. The structure was energy minimized, equilibrated and the random coil conformation was used for running the production simulations. The N-glycosylation, with selected glycan, was added to the asparagine amino acid at residue number 359 using CHARMMGUI web server [70]. Likewise, the phosphorylation was also added through the CHARMMGUI webserver.