Selection of Glycans
Chemical analysis of the different glycans attached to the PHF tau and
AD phosphorylated tau protein obtained from AD brain was carried out by
Sato and coworkers and found that the glycans were rich in High
mannose-type sugar chains and truncated N-glycans were also found in
both tau in addition to a small amount of sialylated bi- and
triantennary sugar chains. Proposed structures and their percent molar
ratios of N-glycans obtained from AD P-tau and PHF-tau were reported by
sato and coworkers [68]. For the purpose of the study, we have
selected the glycan which is present in both PHF tau and AD P-tau, and
at the highest proportion. The structure of the glycan is given in
supporting information (Figure S7 ).