Detection of platelet-specific antibodies
Peripheral blood was collected before the onset of treatment to detect platelet-specific antibodies. Antibodies, anti-GPIIb/IIIa and anti-GPIb/IX, were detected using a commercial kit(PAKAUTO; GTI Diagnostics Inc, Waukesha, WI, USA). The solid-phase modified antigen-capture enzyme-linked immunosorbent assay (ELISA) was employed to detect platelet-specific antibodies 13. The ratio of the patient’s antibody titer to the control’s antibody titer was defined as the antibody titer ratio. We defined the ratio as positive when the antibody titer ratio was >1 in plasma or eluent and defined the ratio as negative when the antibody titer ratio was <1 in both plasma and eluent.