Improved Resolution Fourier Ptychography Scheme Using Oil-Filled
Dome-Shaped LED array
Abstract
Utilizing angular-varied illumination in Fourier Ptychography (FP), the
resolution of optical microscopes can be increased and the phase of the
sample can also be recovered while maintaining a wide field-of-view. In
this work, an FP microscopy imaging setup is demonstrated using a 10X
0.28NA objective lens and a dome-shaped LED array illuminator. By
increasing the refractive index of the medium between the sample and
light sources using oil filled dome, a synthetic system NA of 0.68 is
achieved using only 37 LEDs. The measured resolution of 345 nm at a
wavelength of 530 nm closely matches the theoretical prediction.
Furthermore, the results of the oil-medium illumination are compared to
free-space illumination. The oil immersion on the illumination side
effectively enhances the illumination NA as compared to air and reduces
the need for far off-axis LEDs. The result is high-resolution FP imaging
with relatively fewer LEDs providing both short capture and
reconstruction times.