Soil moisture is an important factor that affects terrestrial vegetation ecosystems and biological growth and development, and water deficit is one of the major environmental factors threatening vegetation restoration in the Loess Plateau. To determine the change in the soil water deficit characteristics from farmland to forestland on the Loess Plateau, in this study, we measured the soil water storage and deficit in abandoned grassland, shrubland, pioneer forestland and climax forestland along with vegetation succession. The results showed that the soil water content and the soil water storage with natural vegetation recovery from the abandoned grassland to shrubland and forestland showed a gradual declining trend, while on the contrary, the soil water deficit was increasing during succession, it was more severe in the deep soil than in the shallow soil layers, and the soil water deficit in July and September of the rainy season as well as that the vigorous growing period of plants was more serious than that in May and November, which are in the non-rainy season of the Chinese Loess Plateau. The vegetation types, soil texture and soil depth were the key factors affecting the soil water deficiency status in the process of vegetation succession. The results could have great potential in the sustainable development of forestry and provide a theoretical basis for effective water management and reasonable vegetation restoration in arid and semiarid loess regions.